Selection and characterization of siderophores of pathogenic <i>Escherichia coli</i> intestinal and extraintestinal isolates

Abstract Microorganisms adopt a unique mechanism for sequestration and transport of bound iron by producing iron-chelating molecules called siderophores. Siderophores play an important role in colonization microbes iron-deficient sites the host, hence acting as virulence factor promising carriers antimicrobials to target inside microbial cells. The research was conducted identify characterize siderophores pathogenic Escherichia coli isolates obtained from different parts India. A total 534 confirmed E. were tested expression using chrome azurol S agar method its type determined Csaky Arnow assays hydroxamate catecholate-type siderophores, respectively. results revealed difference siderophore-type intestinal extraintestinal coli. On 45.1% found produce 12.5% catechol-type siderophore 35.4% produced hydroxamate-type siderophore; could be classified into five sidero-types (1) only (2) catechol (3) both (4) other than types (5) non-producers. Siderophore production detected all geographical regions seasons year. findings conclude that is one epidemiological markers well